Reconstitution of adipokinetic hormone biosynthesis in vitro indicates steps in prohormone processing
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چکیده
منابع مشابه
Adipokinetic hormone-induced lipolysis in the fat
The pathway for the adipokinetic hormone-stiniulated synthesis of sn-1,2-diacylglyceroIs in the adult Manducu sexta fat body was studied. Adult fat body lipids were labeled by feeding 5th instar larvae either with [9,10(n)-3H]oleic acid or [ 1 (3)-3H]glycerol and after 32 days insects at the adult stage were used. This long-term prelabeling led to labeled fat body acylglycerols in which triacyl...
متن کاملIn vitro study of the action of adipokinetic hormone in locusts.
The in vitro study was performed in order to demonstrate the structural changes of lipophorin induced in vivo by the injection of adipokinetic hormone (AKH) into adult locusts. After many unsuccessful attempts, we have established the reconstructed incubation system in which purified lipophorin and apolipophorin-III (9 mol/mol lipophorin) are incubated with the fat body in the presence of AKH u...
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in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
Biosynthesis of adipokinetic hormones (AKHs): further characterization of precursors and identification of novel products of processing.
Two adipokinetic hormones (AKH I: pGlu-Leu-Asn-Phe-Thr-Pro-Asn-Trp-Gly-Thr-NH2, and AKH II: pGlu-Leu-Asn-Phe-Ser-Thr-Gly-Trp-NH2) are synthesized by the neurosecretory cells of the corpora cardiaca (CC) of the locust Schistocerca gregaria. Both AKHs are released into the blood during flight and serve to regulate lipid metabolism and other physiological processes involved in flight. By in vitro ...
متن کامل: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1994
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1994.tb18558.x